I wanted to share some insights and tips on the topic of reconstituting peptides, as it can be a bit tricky and even daunting for those who are new to the peptides world. Proper reconstitution is crucial to ensure the stability and effectiveness of peptides. So, let's discuss the process and some best practices.
Gather Your Supplies:
To get started, make sure you have the necessary supplies ready:
Your lyophilized peptide
Sterile solvent (typically bacteriostatic water)
Syringe (I find a 5 mL syringe works best)
Adhesive labels (sandwich bags also work well to differentiate peptides and keep them clean)
Prepare the Solvent:
Use a sterile solvent, such as bacteriostatic water or sterile saline, to reconstitute your peptide. The volume required is up to your own discretion, however I find that 2mL typically works well.
Step 1: Sterilize your working area and hands properly. Wipe the top of the vial containing the peptide and the solvent with an alcohol swab.
Step 2: Draw up the appropriate amount of solvent into the syringe.
Step 3: Slowly inject the solvent into the vial by aiming the needle at the side of the vial and allowing the liquid to run down the glass. Avoid injecting directly onto the peptide powder to prevent damage.
Step 4: Gently swirl or roll the vial to aid in the dissolution process. Avoid vigorous shaking, as it can lead to peptide degradation or foaming.
Step 5:Allow the vial to rest for a few minutes to ensure complete dissolution. If necessary, gently invert the vial a couple of times to aid in the process.
Storage and Labeling:
Step 1: Once the peptide is fully reconstituted, feel free to administer your first dosage.
Step 2: Label the vial with the peptide name, concentration, date of reconstitution, and any other relevant information. This will help you keep track of your peptides and ensure accurate usage.
Step 3: Store your peptides in the fridge between 2c-8c.
Handling and Usage:
Step 1: When handling reconstituted peptides, follow appropriate safety measures and adhere to established laboratory protocols.
Step 2: Keep track of the expiration date or stability information provided by the manufacturer. Using expired peptides or those that have degraded may compromise experimental results.